Optimization of in vitro regeneration protocol for a popular Indica rice (Oryza sativa L. cv Swarna)
نویسندگان
چکیده
منابع مشابه
Efficient in vitro plant regeneration via leaf base segments of indica rice (Oryza sativa L.).
A reliable and reproducible protocol has been developed for high frequency plant regeneration from 4-5 mm long leaf base segments of 4 days old in vitro germinated seedlings of indica rice (Oryza sativa) cultivar Rasi. The effect of age of seedlings, position of leaf base segments and optimum concentration of 2,4-D on callus induction frequency was investigated with a future aim to use leaf bas...
متن کاملProtocol for in vitro somatic embryogenesis and regeneration of rice (Oryza sativa L.).
Development of highly efficient and reproducible plant regeneration system has tremendous potential to provide improved technology to assist in genetic transformation of indica rice cultivars for their further exploitation in selection. For the development of a highly reproducible regeneration system through somatic embryogenesis, mature embryos of highly popular rice cultivars i.e., Govind (fo...
متن کاملGermination and Plantlet Regeneration of Encapsulated Microshoots of Aromatic Rice (Oryza sativa L. Cv. MRQ 74)
Plant tissues such as somatic embryos, apical shoot tips, axillary shoot buds, embryogenic calli, and protocom-like bodies are potential micropropagules that have been considered for creating synthetic seeds. In the present study, 3-5 mm microshoots of Oryza sativa L. Cv. MRQ 74 were used as explant sources for obtaining synthetic seeds. Microshoots were induced from stem explants on Murashige ...
متن کاملOptimization of ammonium acquisition and metabolism by potassium in rice (Oryza sativa L. cv. IR-72).
ABSTRACT We present the first characterization of K(+) optimization of N uptake and metabolism in an NH(4)(+)-tolerant species, tropical lowland rice (cv. IR-72). (13)N radiotracing showed that increased K(+) supply reduces futile NH(4)(+) cycling at the plasma membrane, diminishing the excessive rates of both unidirectional influx and efflux. Pharmacological testing showed that low-affinity NH...
متن کاملHistology of somatic embryogenesis in rice (Oryza sativa cv. 5272)
Rice (Oryza sativa cv. 5272) embryogenic calli were obtained from mature zygotic embryos culture on Murashige & Skoog (1962) medium supplemented with 2.5 mg/l 2,4dichlorophenoxyacetic acid. Histological analysis of somatic embryogenesis revealed that after two weeks of culture of explants on the callus induction medium, somatic embryo development began with a cluster of proembryogenic cells in ...
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ژورنال
عنوان ژورنال: Annals of Plant Sciences
سال: 2016
ISSN: 2287-688X
DOI: 10.21746/aps.2016.08.002